Romano, S
BAL fluid, cell lysates, or apical culture supernatants were incubated with glutathione peroxidase (Sigma-Aldrich, G6137), cumene hydroperoxide (Sigma-Aldrich, 247502), glutathione reductase (Sigma-Aldrich, G9297), and NADPH (Sigma-Aldrich, N1630) for 30 minutes at 25C
This approach allows for early safety evaluation before broader cohort exposure
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It provides the cysteine residue (the rate-limiting amino acid in glutathione synthesis) and supports endogenous GSH production
Joe Moore: And, um, we really need to like, put up defenses and media and interpersonally to help defend against that because we really dont want to be harming people